- Primate liver and gliadin (GAF-3X) BIOCHIP: antibodies against endomysium and gliadin.
- Detection of antibodies against endomysium and gliadin.
- Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
- Initial dilution 1 : 10; conjugate class anti-human IgA, FITC-labelled.
- Autoantibodies against endomysium react with many types of tissue, e.g. primate oesophagus. The most suitable substrate, however, is primate liver: in the case of a positive sample, filamentous linings of the intralobular sinusoids react.
- With the gliadin (GAF-3X)-coated BIOCHIP, antibodies against gliadin can be analyzed in one and the same test procedure.
- Both anti-endomysium antibodies and antibodies against gliadin (class IgA) are reliable serological markers for an active gluten-sensitive enteropathy. Therefore, their determination can in many cases replace endoscopy and biopsy.
- Incubated ELISA Anti-Gliadin (GAF-3X).
- Monospecific detection of antibodies against gliadin.
- Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
- Serum dilution 1 : 200; conjugate class anti-human IgA or IgG, POD-labelled.
- 3-point calibration. Identical incubation conditions and times: the investigation of IgA and IgG antibodies can be combined without difficulty on one and the same microplate.
- Antigen: Gliadin-analogue fusion peptide (GAF-3X).
- The quantitative determination of antibodies against gliadin is very suitable for monitoring the progress of the disease, compliance with a gluten-free diet, or a gluten tolerance test.
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- Incubated ELISA Anti-Tissue Transglutaminase (Endomysium).
- Monospecific detection of antibodies against tissue transglutaminase.
- Indications: gluten-sensitive enteropathy (celiac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
- Serum dilution 1 : 200; conjugate class anti-human IgA or IgG, POD-labelled.
- 3-point calibration, quantitative.
- Antigen: recombinant, expression with the baculovirus vector in insect cells.
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