Antibodies against Borrelia/TBE Virus
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Indirect Immunofluorescence Test: EUROPLUS™ Anti-Borrelia afzelii, Borrelia burgdorferi, VlsE and OspC Antigen
- Autoantibodies against Borrelia afzelii, VlsE (top), Borrelia burgdorferi and OspC (bottom).
- Sensitive screening test for the detection of anti-Borrelia antibodies.
- Indications: erythema chronicum migrans, lymphadenosis cutis benigna, lymphocytic meningoradiculitis, carditis, arthritis, acrodermatitis chronica atrophicans, neuroborreliosis.
- Initial dilution 1 : 10 (IgM), 1 : 100 (IgG).
- If antibodies against Borrelia afzelii or Borrelia burgdorferi are present, a distinct fluorescence of the bacteria in the smear is obtained.
- With the VlsE or OspC coated BIOCHIPs antibodies against the highly specific and highly sensitive marker antigens VlsE (IgG) or OspC (IgM) can be determined monospecifically in one and the same test procedure. If these antigens fluoresce the antibody result is positive even if the bacteria smears show a negative reaction. Thus, the BIOCHIP Mosaic helps to increase specificity and sensitivity in Borrelia diagnostics.
- The BIOCHIP can be supplemented as required using further substrates, e.g. Borrelia burgdorferi sensu stricto (strains CH or USA) and TBE virus infected cells.
Microplate ELISA: Anti-Borrelia plus VlsE
- Incubated ELISA Anti-Borrelia plus VlsE.
- Sensitive screening test for the detection of anti-Borrelia antibodies.
- Indications: erythema chronicum migrans, lymphadenosis cutis benigna, lymphocytic meningoradiculitis, carditis, arthritis, acrodermatitis chronica atrophicans, neuroborreliosis.
- Serum dilution 1 : 100; conjugate class anti-IgG, anti-IgM or anti-IgAGM (VlsE: -IgG only), POD-labelled.
- 3-point calibration (IgG and IgM). Identical incubation conditions and times: all tests can be combined without difficulty on one and the same microplate.
- Antigens: extract of Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii (whole antigen) / recombinant VlsE from Borrelia burgdorferi sensu stricto. VlsE (variable major protein-like sequence, expressed) is a newly characterized surface protein of Borrelia which is expressed exclusively in vivo and which contains conserved and highly immunogenic epitopes.
- IgM test kit (Anti-Borrelia) includes IgG/RF absorbent in sample buffer for IgG absorption in preparation for the determination of specific IgM class antibodies.
Microplate ELISA: Anti-Borrelia plus VlsE, Antibody Determination in Serum and Cerebrospinal Fluid for Detection of Intrathecal Synthesis of Specific Antibodies against Borrelia
- Table-based evaluation of the CSQrel.
- Antibody determination in serum and cerebrospinal fluid (CSF).
- Indication: Neuroborreliosis.
- CSF dilution 1 : 2, serum dilution 1 : 404; conjugate class anti-IgG, POD-labelled.
- 4-point calibration, quantitative.
- Microplate ELISA for the detection of Borrelia antibodies of class IgM in serum and CSF are also available.
Microplate ELISA: Anti-TBE Virus
- Incubated ELISA Anti-TBE Virus.
- Sensitive screening test for the detection of anti-TBE virus antibodies.
- Indication: Tick-borne encephalitis (TBE).
- Serum dilution 1 : 100; conjugate class anti-IgG or anti-IgM, POD-labelled.
- 3-point calibration (IgG and IgM). Identical incubation conditions and times: both tests can be combined without difficulty on one and the same microplate.
- Antigen: native, strain K23.
- IgM test kit includes IgG/RF absorbent in sample buffer for IgG absorption in preparation for the determination of specific IgM class antibodies.
Anti-Borrelia EUROLINE-RN-AT
- Incubated EUROLINE-WB Anti-Borrelia.
- Specific confirmatory test for the detection of antibodies against Borrelia.
- Indications: erythema chronicum migrans, lymphadenosis cutis benigna, lymphocytic meningoradiculitis, carditis, arthritis, acrodermatitis chronica atrophicans, neuroborreliosis.
- The Anti-Borrelia EUROLINE is coated with both native and recombinant proteins and provides a unique mixture of Borrelia specific antigens: The classical antigens OspC, p83 and p39, which show the highest specificity in their native form, were accurately cut from a Westernblot and applied onto the membrane of the line blot. Five new, recombinant designer antigens (p18, p19, p20, p21, p58) having a very high specificity (IgG) were identified using bioinformatic analysis of the Borrelia genome. For the first time, lipids which have been proven to be immunoreactive and were extracted from the Borrelia membranes, three native OspC antigens (IgM) from B. afzelii, B. burgdorferi and B. garinii and three different VlsE antigens (IgG) from B. afzelii, B. burgdorferi and B. garinii are available on the line blot.
- The serological hit rate is increased by 10% by using three OspC variants in the IgM test.
- Serum dilution 1 : 50; conjugate class anti-IgG or anti-IgM, AP-labelled.
EUROLINE-WB: Anti-Borrelia (Whole Antigen plus VlsE)
- Incubated EUROLINE-WB Anti-Borrelia.
- Specific confirmatory test for the detection of antibodies against Borrelia.
- Indications: erythema chronicum migrans, lymphadenosis cutis benigna, lymphocytic meningoradiculitis, carditis, arthritis, acrodermatitis chronica atrophicans, neuroborreliosis.
- Serum dilution 1 : 50; conjugate class anti-IgG or anti-IgM, AP-labelled.
- Antigens: SDS extract of a Borrelia afzelii strain.
- EUROLINE-WB is a combination of westernblot and line blot techniques. An SDS extract of a Borrelia afzelii strain is electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane. A membrane chip coated with highly purified recombinant VlsE-Antigen is then added to the westernblot strips.
- By additionally determining antibodies against VlsE the serological hit rate can be increased by 20% compared to whole extract Westernblots and by 30% compared to recombinant antigen Westernblots. Of all recombinant antigens, VlsE possesses the highest sensitivity for the detection of a Borrelia infection. Over 85% of IgG-positive sera could be identified at a glance by assessing the VlsE band. VlsE allows detection of antibodies against all Borrelia species, and the risk of a false negative reaction due to species differences is ten times lower.
Westernblot: Anti-Borrelia burgdorferi/B. garinii/B. afzelii
- Incubated Westernblot Anti-Borrelia burgdorferi.
- Specific confirmatory test for the detection of antibodies against the humanpathogen genospecies of Borrelia burgdorferi sensu lato.
- Indications: erythema chronicum migrans, lymphadenosis cutis benigna, lymphocytic meningoradiculitis, carditis, arthritis, acrodermatitis chronica atrophicans, neuroborreliosis.
- Serum dilution 1 : 50; conjugate class anti-IgG or anti-IgM, AP-labelled.
- Antigens: SDS extract of a Borrelia burgdorferi strain.
- Bands from all specific antigens are included and clearly separated.
- Test strips can be automatically incubated and evaluated using the systems EUROBlotMaster und EUROLineScan.
Automated Evaluation of Incubated Membrane Strips with the System EUROLineScan
- The program EUROLineScan from EUROIMMUN has been developed to enable quantitative evaluation of membrane based test systems, facilitate management of data, and provide detailed documentation of results — tasks which have until now required considerable time.
- First, the incubated test strips are scanned using a flatbed scanner or camera system.
- EUROLineScan recognizes the position of the strips, even if they have been placed inexactly, identifies the bands, and measures their intensity. The automated evaluation can be monitored, and it is possible to supplement the data manually.
- The results are then saved together with the image data. It is no longer necessary to archive (potentially infectious) incubated test strips. A separate results sheet can be produced for each patient.
