Antibodies against Helicobacter pylori
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Indirect Immunofluorescence Test: Anti-Helicobacter pylori
- Antibodies against Helicobacter pylori.
- Sensitive screening test for the detection of antibodies against Helicobacter pylori.
- Indications: gastritis, ulcus ventriculi et duodeni. Late consequences: MALT lymphomas and adenocarcinomas.
- Initial dilution 1 : 10 (IgM), 1 : 100 (IgG).
- If antibodies against Helicobacter pylori are present, a distinct fluorescence of the bacteria in the smear is obtained.
- A positive IgA result correlates well with the activity of a gastritis. An increased IgG antibody titer is considered to be a marker for chronic infections. A significant drop in the IgG antibody titer about 6 months after therapy is a sign of success.
- The BIOCHIP can be supplemented as required with further substrates, e.g. other infectious agents or tissue sections of primate stomach.
Microplate ELISA: Anti-Helicobacter pylori
- Incubated ELISA Anti-Helicobacter pylori.
- Sensitive screening test for the detection of antibodies against Helicobacter pylori.
- Indications: gastritis, ulcus ventriculi et duodeni. Late consequences: MALT lymphomas and adenocarcinomas.
- Serum dilution 1 : 100; conjugate class anti-IgA or anti-IgG, POD-labelled.
- Antibodies against Helicobacter pylori antigens can be determined quantitatively in RU/ml.
- 1-point calibration, semi-quantitative (IgA) or 3-point calibration, quantitative (IgG). Identical incubation conditions and times: both tests can be combined without difficulty on one and the same microplate.
- Native antigens.
- IgM test kit includes IgG/RF absorbent in sample buffer for IgG absorption in preparation for the determination of specific IgM class antibodies.
Westernblot: Anti-Helicobacter pylori
- Incubated Westernblot Anti-Helicobacter pylori.
- Specific confirmatory test for the detection of antibodies against Helicobacter pylori.
- Indications: gastritis, ulcus ventriculi et duodeni. Late consequences: MALT lymphomas and adenocarcinomas.
- Serum dilution 1 : 50; conjugate class anti-IgA or anti-IgG, AP-labelled.
- Antigens: antigen extract from a particularly suitable Helicobacter pylori strain.
- Bands from all specific antigens are included and clearly separated.
- Test strips can be automatically incubated and evaluated using the systems EUROBlotMaster und EUROLineScan.
