Antibodies against Herpes-simplex Virus (HSV)
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Indirect Immunofluorescence Test: BIOCHIP Mosaic™ HSV-1/HSV-2
- Sensitive screening test for the detection of antibodies against herpes simplex viruses.
- Indication: herpes simplex.
- Initial dilution 1 : 10 (IgM), 1 : 100 (IgG).
- If antibodies against herpes simplex-2 virus are present in the sample, a typical fluorescence of the infected cells is obtained - mainly in the cytoplasm, less in the cell nuclei.
- As HSV-1 and HSV-2 are morphologically and immunologically closely related, cross-reactions can occur. Differentiation may be attempted by testing a serum against both antigen substrates and comparing the titers.
- The BIOCHIP Mosaic™ can be supplemented as required with further substrates, e.g. other infectious agents.
- Anti-HSV individual tests for indirect immunofluorescence:
Substrate |
| Order No. |
HSV-1 | FI 2531-#### G or M | |
HSV-2 | FI 2532-#### G or M | |
HSV-1 and -2 | FI 2531-####-1 G or M |
Microplate ELISA: Anti-HSV-1, Anti-HSV-2
- Specific confirmatory tests for antibodies against HSV-1 or HSV-2.
- Indication: herpes simplex.
- Serum dilution 1 : 100; conjugate class anti-IgG or anti-IgM, POD-labelled.
- 1-point calibration, semiquantitative (IgM) or 3-point calibration, quantitative (IgG). Identical incubation conditions and times: all tests can be combined without difficulty on one and the same microplate.
- Antigens: type-specific glycoproteins C1 or G2, purified by affinity chromatography. Cross-reactions do not occur.
- IgM test kit includes IgG/RF absorbent in sample buffer for IgG absorption in preparation for the determination of specific IgM class antibodies.
- Available individual ELISA:
Antigen | Order No. | |
HSV-1 |
| EI 2531-9601-2 A, G or M |
HSV-2 | EI 2532-9601-2 A, G or M | |
HSV-1/2-Pool | EI 2531-9601-1 G or M |
EUROLINE-WB: Anti-HSV
- Specific confirmatory test for the differentiation of antibodies against HSV-1 and HSV-2.
- Indication: herpes simplex.
- Serum dilution 1 : 50; conjugate class anti-IgG or anti-IgM, AP-labelled.
- EUROLINE-WB is a combination of westernblot and line blot techniques. Proteins from an SDS extract of HSV-1 are electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane. A membrane chip coated with HSV-2 type-specific glycoprotein G2 (gG 2), purified by affinity chromatography, is then added to the westernblot strips.
- Bands from all specific antigens are included and clearly separated.
- The gG 2 band allows the simple differentiation between HSV-1 and HSV-2 infections.
- Test strips can be automatically incubated and evaluated using the systems EUROBlotMaster und EUROLineScan.
